GFP-Dynamin 2 K44A
(Plasmid #22301)

• Depositing Lab: Pietro De Camilli
• Publication: Ochoa et al J Cell Biol. 2000 Jul 24. 150(2):377-89.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dynamin 2 K44A
• Alt name: Dnm2 K44A
• Species: R. norvegicus (rat)
• Insert Size (bp): 2610
• Mutation: K44A
• GenBank ID: NM_013199
• Entrez Gene: Dnm2 (a.k.a. DYIIAAB)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: Unkown (unknown if destroyed)
• 5′ sequencing primer: EGFP-N Sequencing Primere
• 3′ sequencing primer: EGFP-C Sequencing Primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr Mark McNiven Mayo Clinic
• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry