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pIND4-RC-C2-M210TAG+M252V-oCNF
(Plasmid #222995)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222995 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIND4
  • Backbone size w/o insert (bp) 9238
  • Total vector size (bp) 13251

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    puhA
  • Species
    Rhodobacter sphaeroides
  • Insert Size (bp)
    800
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer AATAGGCGTATCACGAGGCC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    pufL
  • Species
    Rhodobacter sphaeroides
  • Insert Size (bp)
    849

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer TGGGTGTCGAACACGGGCTAC
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    pufM
  • Species
    Rhodobacter sphaeroides
  • Insert Size (bp)
    927
  • Mutation
    changed Tyr M210 to amber stop codon (TAG) for amber suppression and changed Trp M252 to Val

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site NdeI (unknown if destroyed)
  • 5′ sequencing primer GCTGAAGGAAGGCGGGCTG
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    Phe variants PylRs
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    1365
  • Promoter rrnB

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    Mm Pyl tRNA for oF incorporation
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    72
  • Promoter rrnB

Cloning Information for Gene/Insert 5

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIND4-RC-C2-M210TAG+M252V-oCNF was a gift from Steven Boxer (Addgene plasmid # 222995 ; http://n2t.net/addgene:222995 ; RRID:Addgene_222995)
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