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His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
(Plasmid #222991)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222991 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCold
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The Doudna lab uses BL21 Rosetta E. coli for protein expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
  • Alt name
    1x-iGeoCas9(R1W1)-2x
  • Species
    Synthetic
  • Insert Size (bp)
    4992
  • Promoter tac
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • CL7 (N terminal on insert)
    • MBP (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer agcggataacaatttgatgtgc
  • 3′ sequencing primer ATAATACCGCGCCACATAGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star) was a gift from Jennifer Doudna (Addgene plasmid # 222991 ; http://n2t.net/addgene:222991 ; RRID:Addgene_222991)

  • For your References section:

    Lung and liver editing by lipid nanoparticle delivery of a stable CRISPR-Cas9 RNP. Chen K, Han H, Zhao S, Xu B, Yin B, Trinidad M, Burgstone BW, Murthy N, Doudna JA. bioRxiv [Preprint]. 2023 Nov 15:2023.11.15.566339. doi: 10.1101/2023.11.15.566339. 10.1101/2023.11.15.566339 PubMed 38014175
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