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Addgene

GFP-PIPK1 gamma 90
(Plasmid #22299)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22299 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human phosphatidylinositol phosphate kinase type 1 gamma 90
  • Alt name
    PIPK1 gamma 90
  • Alt name
    KIAA0589
  • Alt name
    PtdInsPKI gamma-90
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2100
  • GenBank ID
    NM_012398
  • Entrez Gene
    PIP5K1C (a.k.a. LCCS3, PIP5K-GAMMA, PIP5K1-gamma, PIP5Kgamma)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer EGFP-N Sequencing Primere
  • 3′ sequencing primer EGFP-C Sequencing Primer
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr Nagase Kazusa DNA Research Institute
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-PIPK1 gamma 90 was a gift from Pietro De Camilli (Addgene plasmid # 22299 ; http://n2t.net/addgene:22299 ; RRID:Addgene_22299)

  • For your References section:

    Recruitment and regulation of phosphatidylinositol phosphate kinase type 1 gamma by the FERM domain of talin. Di Paolo G, Pellegrini L, Letinic K, Cestra G, Zoncu R, Voronov S, Chang S, Guo J, Wenk MR, De Camilli P. Nature. 2002 Nov 7. 420(6911):85-9. 10.1038/nature01147 PubMed 12422219
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