pBEL3000
(Plasmid
#222974)
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PurposeTo express a putative signal peptide from Encephalitozoon intestinalis protein Eint_111330 fused to GFP in mammalian cells
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222974 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.4
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesfGFP
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SpeciesPutative signal peptide from Encephalitozoon intestinalis and GFP from Aequorea victoria
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.05.30.596468 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBEL3000 was a gift from Gira Bhabha & Damian Ekiert (Addgene plasmid # 222974 ; http://n2t.net/addgene:222974 ; RRID:Addgene_222974) -
For your References section:
scRNA-seq reveals transcriptional dynamics of Encephalitozoon intestinalis parasites in human macrophages. Jaroenlak P, McCarty KL, Xia B, Lam C, Zwack EE, Yanai I, Bhabha G, Ekiert DC. bioRxiv 2024.05.30.596468 10.1101/2024.05.30.596468