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Addgene

pDEST17_A1LigN_EC
(Plasmid #22296)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22296 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST17
  • Backbone size w/o insert (bp) 6681
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    A1 domain NAD+ dependent DNA ligase N
  • Species
    Escherichia coli
  • Insert Size (bp)
    210
  • Tag / Fusion Protein
    • His tag (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site Gateway (destroyed during cloning)
  • 3′ cloning site Gateway (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST17_A1LigN_EC was a gift from Oscar Millet (Addgene plasmid # 22296 ; http://n2t.net/addgene:22296 ; RRID:Addgene_22296)

  • For your References section:

    Structural basis for the aminoacid composition of proteins from halophilic archea. Tadeo X, Lopez-Mendez B, Trigueros T, Lain A, Castano D, Millet O. PLoS Biol. 2009 Dec;7(12):e1000257. doi: 10.1371/journal.pbio.1000257. Epub 2009 Dec 15. 10.1371/journal.pbio.1000257 PubMed 20016684
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