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Addgene

pQA-LP576
(Plasmid #222955)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222955 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-19b
  • Backbone size w/o insert (bp) 5983
  • Total vector size (bp) 6523
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    antiterminator protein LoaP
  • Species
    Thermoanaerobacter pseudethanolicus ATCC 33223
  • Insert Size (bp)
    540
  • Mutation
    mutated Arginine 141 to Serine
  • GenBank ID
    ABY95203.1
  • Promoter T7
  • Tag / Fusion Protein
    • 10xHIS-SUMO (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQA-LP576 was a gift from Adrian Ferré-D'Amaré (Addgene plasmid # 222955 ; http://n2t.net/addgene:222955 ; RRID:Addgene_222955)

  • For your References section:

    Major-groove sequence-specific RNA recognition by LoaP, a paralog of transcription elongation factor NusG. Elghondakly A, Jermain MD, Winkler WC, Ferre-D'Amare AR. Structure. 2024 Jun 28:S0969-2126(24)00219-3. doi: 10.1016/j.str.2024.06.001. 10.1016/j.str.2024.06.001 PubMed 38959899
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