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Addgene

PB-NES-ZapCV2
(Plasmid #222950)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222950 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Piggyback
  • Backbone manufacturer
    Genevie
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NES-ZapCV2
  • Species
    Synthetic
  • Insert Size (bp)
    1693
  • Mutation
    ECFP gene is truncated 36 bp at 3' end. Venus gene is circularly permuted at residue 173. Zap2 zinc binding domain derived from S. cerevisiae Zap1 zinc finger domains 1 & 2 with C581H and C618H mutations
  • Entrez Gene
    NES (a.k.a. Nbla00170)
  • Tags / Fusion Proteins
    • Nuclear Export Signal (NES) derived from HIV-1 (N terminal on insert)
    • ECFP (Enhanced Cyan Fluorescent Protein) (N terminal on insert)
    • cp173Venus (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpnl (not destroyed)
  • 3′ cloning site ECoRI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer l-12EndR
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositors also recommend growing this plasmids in Omnimax cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-NES-ZapCV2 was a gift from Amy Palmer (Addgene plasmid # 222950 ; http://n2t.net/addgene:222950 ; RRID:Addgene_222950)

  • For your References section:

    Droplet Microfluidic Flow Cytometer For Sorting On Transient Cellular Responses Of Genetically-Encoded Sensors. Fiedler BL, Van Buskirk S, Carter KP, Qin Y, Carpenter MC, Palmer AE, Jimenez R. Anal Chem. 2017 Jan 3;89(1):711-719. doi: 10.1021/acs.analchem.6b03235. Epub 2016 Dec 13. 10.1021/acs.analchem.6b03235 PubMed 27959493
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