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Addgene

pcDNA-CAML-Myc-His
(Plasmid #22295)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22295 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/myc-his(-)
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    calcium modulating ligand
  • Alt name
    CAMLG, CAML,
  • Alt name
    integral membrane protein; calcium-modulating cyclophilin ligand; calcium-signal modulating cyclophilin ligand
  • Alt name
    cyclophilin B-binding protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    903
  • GenBank ID
    NM_001745
  • Entrez Gene
    CAMLG (a.k.a. CAML, GET2)
  • Tag / Fusion Protein
    • Myc-His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho I (not destroyed)
  • 3′ cloning site BamH I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-CAML-Myc-His was a gift from Erik Lillehoj (Addgene plasmid # 22295 ; http://n2t.net/addgene:22295 ; RRID:Addgene_22295)

  • For your References section:

    MUC1 mucin interacts with calcium-modulating cyclophilin ligand. Guang W, Kim KC, Lillehoj EP. Int J Biochem Cell Biol. 2009 Jun . 41(6):1354-60. 10.1016/j.biocel.2008.12.004 PubMed 19135167
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