2COX8-BmTyr
(Plasmid
#222943)
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Purposemitochondrion-localized BmTyr
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222943 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N
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Modifications to backboneEGFP was omitted
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name2COX8-BmTyr-Flag
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GenBank ID
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Tag
/ Fusion Protein
- Flag tag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
2COX8-BmTyr was a gift from Itaru Hamachi (Addgene plasmid # 222943 ; http://n2t.net/addgene:222943 ; RRID:Addgene_222943) -
For your References section:
Tyrosinase-Based Proximity Labeling in Living Cells and In Vivo. Zhu H, Oh JH, Matsuda Y, Mino T, Ishikawa M, Nakamura H, Tsujikawa M, Nonaka H, Hamachi I. J Am Chem Soc. 2024 Mar 20;146(11):7515-7523. doi: 10.1021/jacs.3c13183. Epub 2024 Mar 6. 10.1021/jacs.3c13183 PubMed 38445591