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Addgene

mCherry-BmTyr-H2B
(Plasmid #222941)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222941 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BmTyr-H2B
  • GenBank ID
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrG1 (for BmTyr), XhoI (for H2B) (not destroyed)
  • 3′ cloning site XhoI (for BmTyr), HindIII (for H2B) (not destroyed)
  • 5′ sequencing primer GAACAGTACGAACGCGCC
  • 3′ sequencing primer GTTCAGGGGGAGGTGTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-BmTyr-H2B was a gift from Itaru Hamachi (Addgene plasmid # 222941 ; http://n2t.net/addgene:222941 ; RRID:Addgene_222941)

  • For your References section:

    Tyrosinase-Based Proximity Labeling in Living Cells and In Vivo. Zhu H, Oh JH, Matsuda Y, Mino T, Ishikawa M, Nakamura H, Tsujikawa M, Nonaka H, Hamachi I. J Am Chem Soc. 2024 Mar 20;146(11):7515-7523. doi: 10.1021/jacs.3c13183. Epub 2024 Mar 6. 10.1021/jacs.3c13183 PubMed 38445591
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