pSrRGECO1
(Plasmid
#222940)
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PurposeExpresses RGECO1 (fluorescent calcium indicator) and pac (puromycin resistance) in the Choanoflagellate, Salpingoeca rosetta
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222940 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneNK802
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Backbone manufacturerNicole King
- Backbone size w/o insert (bp) 6293
- Total vector size (bp) 6833
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Modifications to backbonemCherry sequence removed (714bp)
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Vector typeSalpingoeca rosetta expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRGECO1
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SpeciesSynthetic
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Insert Size (bp)1253
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.06.19.599169 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSrRGECO1 was a gift from Pawel Burkhardt (Addgene plasmid # 222940 ; http://n2t.net/addgene:222940 ; RRID:Addgene_222940) -
For your References section:
Electrical signaling and coordinated behavior in the closest relative of animals. Colgren J, Burkhardt P. Sci Adv. 2025 Jan 10;11(2):eadr7434. doi: 10.1126/sciadv.adr7434. Epub 2025 Jan 8. 10.1126/sciadv.adr7434 PubMed 39772683