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Addgene

pOSV00157
(Plasmid #222937)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222937 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1
  • Vector type
    Bacterial Expression
  • Selectable markers
    Spectinomycin resistance gene for Bacillus subtilis genomic integration at amyE locus

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The leaky expression of the toxin on the plasmid could lead to outgrowing mutants. It is suggested that a few colonies can be streaked for growth and plasmid extraction. The plasmid extraction should be done before the OD gets too high.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TxpA-RatA toxin-antitoxin
  • Species
    Bacillus subtilis
  • Promoter Phyperspank

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOSV00157 was a gift from Ophelia Venturelli (Addgene plasmid # 222937 ; http://n2t.net/addgene:222937 ; RRID:Addgene_222937)

  • For your References section:

    Programming bacteria for multiplexed DNA detection. Cheng YY, Chen Z, Cao X, Ross TD, Falbel TG, Burton BM, Venturelli OS. Nat Commun. 2023 Apr 10;14(1):2001. doi: 10.1038/s41467-023-37582-x. 10.1038/s41467-023-37582-x PubMed 37037805
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