pEGFPC1-FLAG-Synaptojanin 1-145
(Plasmid #22293)

• Depositing Lab: Pietro De Camilli
• Publication: Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 103(51):19332-7.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Synaptojanin 1_145
• Alt name: inositol 5'-phosphatase p145 isoform
• Species: H. sapiens (human)
• Insert Size (bp): 4500
• GenBank ID: AF009039
• Entrez Gene: SYNJ1 (a.k.a. DEE53, EIEE53, INPP5G, PARK20)
• Tags / Fusion Proteins:
  • GFP (N terminal on backbone)
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: EGFP-N Sequencing Primer
• 3′ sequencing primer: EGFP-C Sequencing Primer

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry