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pcDNA3-FLAG-Synaptojanin 1-170
(Plasmid #22292)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22292 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Synaptojanin 1_170
  • Alt name
    inositol 5'-phosphatase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4500
  • Mutation
    K334R compared to Genbank ID NM_003895
  • GenBank ID
    NM_003895
  • Entrez Gene
    SYNJ1 (a.k.a. DEE53, EIEE53, INPP5G, PARK20)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Compared to Genbank ID NM_003895, synaptojanin has a K334R mutation. However, this mutation is present in the known variant: GenBank ID AAC51922.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-FLAG-Synaptojanin 1-170 was a gift from Pietro De Camilli (Addgene plasmid # 22292 ; http://n2t.net/addgene:22292 ; RRID:Addgene_22292)

  • For your References section:

    Synaptojanin 1: localization on coated endocytic intermediates in nerve terminals and interaction of its 170 kDa isoform with Eps15. Haffner C, Takei K, Chen H, Ringstad N, Hudson A, Butler MH, Salcini AE, Di Fiore PP, De Camilli P. FEBS Lett. 1997 Dec 15. 419(2-3):175-80. 10.1016/S0014-5793(97)01451-8 PubMed 9428629
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