pHDR2_TFAP2C-T2A-mGreenLantern-Hygro
(Plasmid
#222915)
-
PurposeHomology directed repair template for knocking in mGreenLantern reporter to TFAP2C.
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 222915 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepHDR
-
Vector typeCRISPR
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemGreenLantern
-
SpeciesH. sapiens (human), Synthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2022.07.11.499564 for bioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHDR2_TFAP2C-T2A-mGreenLantern-Hygro was a gift from George Church (Addgene plasmid # 222915 ; http://n2t.net/addgene:222915 ; RRID:Addgene_222915) -
For your References section:
Rapid Human Oogonia-like Cell Specification via Combinatorial Transcription Factor-Directed Differentiation. Pierson Smela M, Kramme CC, Fortuna PRJ, Wolf B, Srikar Kavirayuni V, Adams J, Ma C, Velychko S, Widocki U, Goel S, Chen T, Vincoff S, Dong E, Kohman RE, Kobayashi M, Shioda T, Church GM, Chatterjee P. bioRxiv 2022.07.11.499564 10.1101/2022.07.11.499564