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L2383-FRB-GPA-NLuc
(Plasmid #222902)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222902 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Modified pcDNA3.1(+)
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 5370
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FRB-GPA-20GS-NanoLuc
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1356
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.12.16.571988 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L2383-FRB-GPA-NLuc was a gift from Joshua Leonard (Addgene plasmid # 222902 ; http://n2t.net/addgene:222902 ; RRID:Addgene_222902)

  • For your References section:

    Building Synthetic Biosensors Using Red Blood Cell Proteins. Dolberg TB, Gunnels TF, Ling T, Sarnese KA, Crispino JD, Leonard JN. ACS Synth Biol. 2024 Apr 19;13(4):1273-1289. doi: 10.1021/acssynbio.3c00754. Epub 2024 Mar 27. 10.1021/acssynbio.3c00754 PubMed 38536408
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