L2380-FKBP-GPA(V84R)-20-114
(Plasmid
#222900)
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PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222900 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneModified pcDNA3.1(+)
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Backbone manufacturerThermo Fisher
- Backbone size w/o insert (bp) 5344
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFKBP-GPA(V84R)-20GS-NanoLuc114
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)1079
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MutationV84R in GPA
- Promoter CMV
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NotI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.12.16.571988 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
L2380-FKBP-GPA(V84R)-20-114 was a gift from Joshua Leonard (Addgene plasmid # 222900 ; http://n2t.net/addgene:222900 ; RRID:Addgene_222900) -
For your References section:
Building Synthetic Biosensors Using Red Blood Cell Proteins. Dolberg TB, Gunnels TF, Ling T, Sarnese KA, Crispino JD, Leonard JN. ACS Synth Biol. 2024 Apr 19;13(4):1273-1289. doi: 10.1021/acssynbio.3c00754. Epub 2024 Mar 27. 10.1021/acssynbio.3c00754 PubMed 38536408