pR5-19
(Plasmid
#22288)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22288 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneYCplac111
- Backbone size w/o insert (bp) 6112
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRAD5
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Alt namerad5-Ub ligase mutant
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)3510
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MutationC914, C917->AA in the C3HC4 ring-finger motif
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Entrez GeneRAD5 (a.k.a. YLR032W, REV2, SNM2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13_pUC-rev
- 3′ sequencing primer M13_puc-for (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mutation abolishes the ubiquitin ligase function
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pR5-19 was a gift from Louise Prakash (Addgene plasmid # 22288 ; http://n2t.net/addgene:22288 ; RRID:Addgene_22288) -
For your References section:
Requirement of Rad5 for DNA polymerase zeta-dependent translesion synthesis in Saccharomyces cerevisiae. Pages V, Bresson A, Acharya N, Prakash S, Fuchs RP, Prakash L. Genetics. 2008 Sep . 180(1):73-82. 10.1534/genetics.108.091066 PubMed 18757916