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Addgene

L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135
(Plasmid #222875)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222875 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV
  • Backbone manufacturer
    Takara
  • Backbone size w/o insert (bp) 5093
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FRB-GPA(WT)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    3784
  • Promoter MSCV LTR
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site ClaI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.12.16.571988 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135 was a gift from Joshua Leonard (Addgene plasmid # 222875 ; http://n2t.net/addgene:222875 ; RRID:Addgene_222875)

  • For your References section:

    Building Synthetic Biosensors Using Red Blood Cell Proteins. Dolberg TB, Gunnels TF, Ling T, Sarnese KA, Crispino JD, Leonard JN. ACS Synth Biol. 2024 Apr 19;13(4):1273-1289. doi: 10.1021/acssynbio.3c00754. Epub 2024 Mar 27. 10.1021/acssynbio.3c00754 PubMed 38536408
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