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Addgene

pRSF-G1(5CNW)RS
(Plasmid #222872)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222872 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSF
  • Backbone size w/o insert (bp) 2452
  • Total vector size (bp) 3274
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tRNA synthetase
  • Alt name
    G1(5CNW)RS
  • Species
    Methanogenic archaeon ISO4-G1
  • Insert Size (bp)
    822
  • Promoter T7

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSF-G1(5CNW)RS was a gift from Thomas Huber (Addgene plasmid # 222872 ; http://n2t.net/addgene:222872 ; RRID:Addgene_222872)

  • For your References section:

    Rendering Proteins Fluorescent Inconspicuously: Genetically Encoded 4-Cyanotryptophan Conserves Their Structure and Enables the Detection of Ligand Binding Sites. Qianzhu H, Abdelkader EH, Welegedara AP, Habel E, Paul N, Frkic RL, Jackson CJ, Huber T, Otting G. Angew Chem Int Ed Engl. 2024 Dec 4:e202421000. doi: 10.1002/anie.202421000. 10.1002/anie.202421000 PubMed 39632265
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