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Addgene

pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA
(Plasmid #222861)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222861 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418) ; Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OgeuIscB Nickase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1485
  • Mutation
    E193A for nickase mutation
  • Promoter CMV
  • Tags / Fusion Proteins
    • ITR2 (N terminal on insert)
    • 3X HA and Nucleoplasmin NLS (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original plasmid is pHS0810 (Addgene #176540). The E193A mutation is the only difference with the pHS0810 plasmid.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.10.24.563270 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA was a gift from Vincent Dion (Addgene plasmid # 222861 ; http://n2t.net/addgene:222861 ; RRID:Addgene_222861)

  • For your References section:

    Developing small Cas9 hybrids using molecular modeling. Mangin A, Dion V, Menzies G. bioRxiv 2023.10.24.563270 10.1101/2023.10.24.563270
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