hIR-GFP
(Plasmid #22286)

• Depositing Lab: Joseph Bass
• Publication: Ramos et al Proc Natl Acad Sci U S A. 2007 Jun 19. 104(25):10470-5.

Backbone

• Vector backbone: pEGFP-N2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: INSR insulin receptor
• Alt name: IR
• Species: H. sapiens (human)
• Insert Size (bp): 4700
• GenBank ID: M10051
• Entrez Gene: INSR (a.k.a. CD220, HHF5)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (destroyed during cloning)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: ACG CAA ATG GGC GGT AGG CG
• 3′ sequencing primer: TCG CCG GAC ACG CTG AAC TTG TG

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry