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pUC57-ITR2-H1-sgAGC-SlugD10A-3XFLAG-SV40 NLS-ITR2
(Plasmid #222859)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222859 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Slug-KHD10A+sgAGC
  • gRNA/shRNA sequence
    GAGCAGCAGCAGCAGCAGC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4000
  • Mutation
    KH variant and D10A mutation
  • Promoter H1 and eCMV
  • Tags / Fusion Proteins
    • ITR2 (N terminal on insert)
    • 3X FLAG, SV40 NLS, ITR2 (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.10.24.563270 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-ITR2-H1-sgAGC-SlugD10A-3XFLAG-SV40 NLS-ITR2 was a gift from Vincent Dion (Addgene plasmid # 222859 ; http://n2t.net/addgene:222859 ; RRID:Addgene_222859)

  • For your References section:

    Developing small Cas9 hybrids using molecular modeling. Mangin A, Dion V, Menzies G. bioRxiv 2023.10.24.563270 10.1101/2023.10.24.563270
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