pCDNA3.1_Zeo_TetO_FLAG-AT1R
(Plasmid
#222856)
-
PurposeMammalian expression plasmid for FLAG-tagged AT1R
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 222856 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCDNA3.1_Zeo_TetO
-
Vector typeMammalian Expression
-
Selectable markersZeocin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameAT1R
-
SpeciesH. sapiens (human)
-
Entrez GeneAGTR1 (a.k.a. AG2S, AGTR1B, AT1, AT1AR, AT1B, AT1BR, AT1R, AT2R1, ATR1, HAT1R)
-
Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional references:
McMahon C, Staus DP, Wingler LM, Wang J, Skiba MA, Elgeti M, Hubbell WL, Rockman HA, Kruse AC, Lefkowitz RJ. Synthetic nanobodies as angiotensin receptor blockers. Proc Natl Acad Sci U S A. 2020 Aug 18;117(33):20284-20291. doi: 10.1073/pnas.2009029117. Epub 2020 Aug 4. PMID: 32753386; PMCID: PMC7443875.
Wingler LM, McMahon C, Staus DP, Lefkowitz RJ, Kruse AC. Distinctive Activation Mechanism for Angiotensin Receptor Revealed by a Synthetic Nanobody. Cell. 2019 Jan 24;176(3):479-490.e12. doi: 10.1016/j.cell.2018.12.006. Epub 2019 Jan 10. PMID: 30639100; PMCID: PMC6367718.
Wingler LM, Skiba MA, McMahon C, Staus DP, Kleinhenz ALW, Suomivuori CM, Latorraca NR, Dror RO, Lefkowitz RJ, Kruse AC. Angiotensin and biased analogs induce structurally distinct active conformations within a GPCR. Science. 2020 Feb 21;367(6480):888-892. doi: 10.1126/science.aay9813. PMID: 32079768; PMCID: PMC7171558.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCDNA3.1_Zeo_TetO_FLAG-AT1R was a gift from Andrew Kruse (Addgene plasmid # 222856 ; http://n2t.net/addgene:222856 ; RRID:Addgene_222856) -
For your References section:
Antibodies expand the scope of angiotensin receptor pharmacology. Skiba MA, Sterling SM, Rawson S, Zhang S, Xu H, Jiang H, Nemeth GR, Gilman MSA, Hurley JD, Shen P, Staus DP, Kim J, McMahon C, Lehtinen MK, Rockman HA, Barth P, Wingler LM, Kruse AC. Nat Chem Biol. 2024 May 14. doi: 10.1038/s41589-024-01620-6. 10.1038/s41589-024-01620-6 PubMed 38744986