pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG
(Plasmid
#222854)
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PurposeMammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 Fc
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222854 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFUSE-CHIg-hG1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAT118-L
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SpeciesSynthetic
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MutationL234A, L235A, P329G
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Tag
/ Fusion Protein
- IgG1_Fc (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG was a gift from Andrew Kruse (Addgene plasmid # 222854 ; http://n2t.net/addgene:222854 ; RRID:Addgene_222854) -
For your References section:
Antibodies expand the scope of angiotensin receptor pharmacology. Skiba MA, Sterling SM, Rawson S, Zhang S, Xu H, Jiang H, Nemeth GR, Gilman MSA, Hurley JD, Shen P, Staus DP, Kim J, McMahon C, Lehtinen MK, Rockman HA, Barth P, Wingler LM, Kruse AC. Nat Chem Biol. 2024 May 14. doi: 10.1038/s41589-024-01620-6. 10.1038/s41589-024-01620-6 PubMed 38744986