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Addgene

pET26b_AT118-H-V5-His
(Plasmid #222850)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222850 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET26b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AT118-H
  • Species
    Synthetic
  • Tag / Fusion Protein
    • V5-His (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional reference:
McMahon C, Staus DP, Wingler LM, Wang J, Skiba MA, Elgeti M, Hubbell WL, Rockman HA, Kruse AC, Lefkowitz RJ. Synthetic nanobodies as angiotensin receptor blockers. Proc Natl Acad Sci U S A. 2020 Aug 18;117(33):20284-20291. doi: 10.1073/pnas.2009029117. Epub 2020 Aug 4. PMID: 32753386; PMCID: PMC7443875.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET26b_AT118-H-V5-His was a gift from Andrew Kruse (Addgene plasmid # 222850 ; http://n2t.net/addgene:222850 ; RRID:Addgene_222850)

  • For your References section:

    Antibodies expand the scope of angiotensin receptor pharmacology. Skiba MA, Sterling SM, Rawson S, Zhang S, Xu H, Jiang H, Nemeth GR, Gilman MSA, Hurley JD, Shen P, Staus DP, Kim J, McMahon C, Lehtinen MK, Rockman HA, Barth P, Wingler LM, Kruse AC. Nat Chem Biol. 2024 May 14. doi: 10.1038/s41589-024-01620-6. 10.1038/s41589-024-01620-6 PubMed 38744986
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