pYDS2.0_AT118-H
(Plasmid
#222846)
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PurposeYeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222846 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYDS2.0
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAT118-H
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional reference:
McMahon C, Staus DP, Wingler LM, Wang J, Skiba MA, Elgeti M, Hubbell WL, Rockman HA, Kruse AC, Lefkowitz RJ. Synthetic nanobodies as angiotensin receptor blockers. Proc Natl Acad Sci U S A. 2020 Aug 18;117(33):20284-20291. doi: 10.1073/pnas.2009029117. Epub 2020 Aug 4. PMID: 32753386; PMCID: PMC7443875.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYDS2.0_AT118-H was a gift from Andrew Kruse (Addgene plasmid # 222846 ; http://n2t.net/addgene:222846 ; RRID:Addgene_222846) -
For your References section:
Antibodies expand the scope of angiotensin receptor pharmacology. Skiba MA, Sterling SM, Rawson S, Zhang S, Xu H, Jiang H, Nemeth GR, Gilman MSA, Hurley JD, Shen P, Staus DP, Kim J, McMahon C, Lehtinen MK, Rockman HA, Barth P, Wingler LM, Kruse AC. Nat Chem Biol. 2024 May 14. doi: 10.1038/s41589-024-01620-6. 10.1038/s41589-024-01620-6 PubMed 38744986