pcDNA-HA-CAML
(Plasmid #22284)

• Depositing Lab: Erik Lillehoj
• Publication: Guang et al Int J Biochem Cell Biol. 2009 Jun . 41(6):1354-60.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: calcium-modulating ligand
• Alt name: CAMLG, CAML,
• Alt name: integral membrane protein; calcium-modulating cyclophilin ligand; calcium-signal modulating cyclophilin ligand
• Alt name: cyclophilin B-binding protein
• Species: H. sapiens (human)
• Insert Size (bp): 938
• GenBank ID: NM_001745
• Entrez Gene: CAMLG (a.k.a. CAML, GET2)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho I (not destroyed)
• 3′ cloning site: BamH I (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry