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Addgene

pQCXIB CMV/TO DsRed2 (w444-1)
(Plasmid #22274)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22274 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIB
  • Backbone size w/o insert (bp) 7025
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Top10F', 37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Discosoma sp. red fluorescent protein 2
  • Alt name
    DsRed2
  • Insert Size (bp)
    677

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer CMVforw
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DsRed2 cDNA was obtained from Dr. Christian Beausejour.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIB CMV/TO DsRed2 (w444-1) was a gift from Eric Campeau (Addgene plasmid # 22274 ; http://n2t.net/addgene:22274 ; RRID:Addgene_22274)
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