pNP2270
(Plasmid
#222733)
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PurposepNP1950 - J23119-IS621 bRNA1 TBL-lacZ-1 (Extended RTG), DBL-WT, HDV; T7-IS621 Recombinase
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 222733 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNP1950 - p15A Ori
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameIS621 Helper (lacZ)
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SpeciesE. coli
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.01.24.577089 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNP2270 was a gift from Patrick Hsu (Addgene plasmid # 222733 ; http://n2t.net/addgene:222733 ; RRID:Addgene_222733) -
For your References section:
Bridge RNAs direct programmable recombination of target and donor DNA. Durrant MG, Perry NT, Pai JJ, Jangid AR, Athukoralage JS, Hiraizumi M, McSpedon JP, Pawluk A, Nishimasu H, Konermann S, Hsu PD. Nature. 2024 Jun;630(8018):984-993. doi: 10.1038/s41586-024-07552-4. Epub 2024 Jun 26. 10.1038/s41586-024-07552-4 PubMed 38926615
