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Addgene

pNP1954
(Plasmid #222730)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222730 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNP1723 - ColE1 Ori
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    IS621 bridge RNA
  • Species
    E. coli
  • Tag / Fusion Protein
    • sfGFP (N terminal on backbone)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.01.24.577089 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNP1954 was a gift from Patrick Hsu (Addgene plasmid # 222730 ; http://n2t.net/addgene:222730 ; RRID:Addgene_222730)

  • For your References section:

    Bridge RNAs direct programmable recombination of target and donor DNA. Durrant MG, Perry NT, Pai JJ, Jangid AR, Athukoralage JS, Hiraizumi M, McSpedon JP, Pawluk A, Nishimasu H, Konermann S, Hsu PD. Nature. 2024 Jun;630(8018):984-993. doi: 10.1038/s41586-024-07552-4. Epub 2024 Jun 26. 10.1038/s41586-024-07552-4 PubMed 38926615
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