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pRSFDuet-HIS-FLAG-AtUBQ11
(Plasmid #222720)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222720 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDuet-1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AtUBQ11
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    UBQ11 (a.k.a. AT4G05050, C17L7.6, ubiquitin 11)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • 2xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer GGATCTCGACGCTCTCCCT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSFDuet-HIS-FLAG-AtUBQ11 was a gift from Lei Wu (Addgene plasmid # 222720 ; http://n2t.net/addgene:222720 ; RRID:Addgene_222720)

  • For your References section:

    A Bacterial Platform for Studying Ubiquitination Cascades Anchored by SCF-Type E3 Ubiquitin Ligases. Pu ZX, Wang JL, Li YY, Liang LY, Tan YT, Wang ZH, Li BL, Guo GQ, Wang L, Wu L. Biomolecules. 2024 Sep 25;14(10):1209. doi: 10.3390/biom14101209. 10.3390/biom14101209 PubMed 39456142
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