pETDuet-AtRBX1-T7-AtASK1-T7-AtCUL1-S
(Plasmid #222715)

• Purpose: Expression AtRBX1-T7, AtASK1-T7 and AtCUL1-S in in bacterial cells
• Depositing Lab: Lei Wu
• Publication: Pu et al Biomolecules. 2024 Sep 25;14(10):1209. doi: 10.3390/biom14101209.

Backbone

• Vector backbone: pETDuet-1
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: AtRBX1
• Species: A. thaliana (mustard weed)
• Entrez Gene: RBX1 (a.k.a. AT5G20570, ATRBX1, F7C8.160, F7C8_160, HRT1, REGULATOR OF CULLINS-1, RING-BOX 1, RING-box 1, ROC1)
• Tag / Fusion Protein:
  • T7 (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: AtASK1
• Species: A. thaliana (mustard weed)
• Entrez Gene: SKP1 (a.k.a. AT1G75950, ARABIDOPSIS SKP1 HOMOLOGUE 1, ASK1, ATSKP1, S phase kinase-associated protein 1, SKP1A, T4O12.17, T4O12_17, UFO INTERACTING PROTEIN 1, UIP1)
• Tag / Fusion Protein:
  • T7 (C terminal on insert)

Gene/Insert 3

• Gene/Insert name: AtCUL1
• Species: A. thaliana (mustard weed)
• Entrez Gene: CUL1 (a.k.a. AT4G02570, ATCUL1, AUXIN RESISTANT 6, AXR6, T10P11.26, T10P11_26, cullin 1)
• Tag / Fusion Protein:
  • S-Tag (C terminal on backbone)

Resource Information

• Supplemental Documents:
  • pETDuet-AtRBX1-T7-AtASK1-T7-AtCUL1-S.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry