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pETDuet-AtRBX1-T7-AtASK1-T7-AtCUL1-S
(Plasmid #222715)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222715 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETDuet-1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    AtRBX1
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    RBX1 (a.k.a. AT5G20570, ATRBX1, F7C8.160, F7C8_160, HRT1, REGULATOR OF CULLINS-1, RING-BOX 1, RING-box 1, ROC1)
  • Tag / Fusion Protein
    • T7 (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    AtASK1
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    SKP1 (a.k.a. AT1G75950, ARABIDOPSIS SKP1 HOMOLOGUE 1, ASK1, ATSKP1, S phase kinase-associated protein 1, SKP1A, T4O12.17, T4O12_17, UFO INTERACTING PROTEIN 1, UIP1)
  • Tag / Fusion Protein
    • T7 (C terminal on insert)

Gene/Insert 3

  • Gene/Insert name
    AtCUL1
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    CUL1 (a.k.a. AT4G02570, ATCUL1, AUXIN RESISTANT 6, AXR6, T10P11.26, T10P11_26, cullin 1)
  • Tag / Fusion Protein
    • S-Tag (C terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDuet-AtRBX1-T7-AtASK1-T7-AtCUL1-S was a gift from Lei Wu (Addgene plasmid # 222715 ; http://n2t.net/addgene:222715 ; RRID:Addgene_222715)

  • For your References section:

    A Bacterial Platform for Studying Ubiquitination Cascades Anchored by SCF-Type E3 Ubiquitin Ligases. Pu ZX, Wang JL, Li YY, Liang LY, Tan YT, Wang ZH, Li BL, Guo GQ, Wang L, Wu L. Biomolecules. 2024 Sep 25;14(10):1209. doi: 10.3390/biom14101209. 10.3390/biom14101209 PubMed 39456142
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