pCDFDuet-AtUBC8-S-AtUBA1-S
(Plasmid
#222714)
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PurposeExpression AtUBA1-S and AtUBC8-S in in bacterial cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222714 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDFDuet-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameAtUBC8
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SpeciesA. thaliana (mustard weed)
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Entrez GeneUBC8 (a.k.a. AT5G41700, ARABIDOPSIS THALIANA UBIQUITIN CONJUGATING ENZYME 8, ATUBC8, MBK23.24, MBK23_24, UBIQUITIN CONJUGATING ENZYME, ubiquitin conjugating enzyme 8)
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Tag
/ Fusion Protein
- S-Tag (C terminal on insert)
Gene/Insert 2
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Gene/Insert nameATUBA1
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SpeciesA. thaliana (mustard weed)
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Entrez GeneUBA1 (a.k.a. AT2G30110, ATUBA1, MODIFIER OF SNC1 5, MOS5, T27E13.15, T27E13_15, ubiquitin-activating enzyme 1)
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Tag
/ Fusion Protein
- S-Tag (C terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDFDuet-AtUBC8-S-AtUBA1-S was a gift from Lei Wu (Addgene plasmid # 222714 ; http://n2t.net/addgene:222714 ; RRID:Addgene_222714) -
For your References section:
A Bacterial Platform for Studying Ubiquitination Cascades Anchored by SCF-Type E3 Ubiquitin Ligases. Pu ZX, Wang JL, Li YY, Liang LY, Tan YT, Wang ZH, Li BL, Guo GQ, Wang L, Wu L. Biomolecules. 2024 Sep 25;14(10):1209. doi: 10.3390/biom14101209. 10.3390/biom14101209 PubMed 39456142