SV40 Large T K1 mutant in pENTR1A (w414-1)
(Plasmid #22270)

• Depositing Lab: Eric Campeau
• Publication: shMDC1 plasmids (unpublished)

Backbone

• Vector backbone: pENTR1A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2279
• Vector type: Entry vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: TOP10F', 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SV40 Large T antigen TK1 mutant (E107K)
• Alt name: TK1
• Insert Size (bp): 2126
• Mutation: E107K (K1 mutation)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bam HI (destroyed during cloning)
• 3′ cloning site: Xho I (destroyed during cloning)
• 5′ sequencing primer: ENTRforw
• 3′ sequencing primer: ENTRrev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Large T K1 was obtained from Dr. Francis Rodier.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Large T antigen K1 mutant can no longer bind and inactivate the Rb, p130 and p107 proteins (DeCaprio et al. 1988, Stubdal et al. 1997). It also fails to activate Akt (Yu and Alwine 2008).