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Addgene

pLenti-UMI
(Plasmid #222694)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222694 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUW
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Neomycin (select with G418) ; GFP
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Mutation
    PGK without BsmBI cutsite
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-UMI was a gift from Ulrich Elling (Addgene plasmid # 222694 ; http://n2t.net/addgene:222694 ; RRID:Addgene_222694)

  • For your References section:

    CRISPR-UMI: single-cell lineage tracing of pooled CRISPR-Cas9 screens. Michlits G, Hubmann M, Wu SH, Vainorius G, Budusan E, Zhuk S, Burkard TR, Novatchkova M, Aichinger M, Lu Y, Reece-Hoyes J, Nitsch R, Schramek D, Hoepfner D, Elling U. Nat Methods. 2017 Dec;14(12):1191-1197. doi: 10.1038/nmeth.4466. Epub 2017 Oct 16. 10.1038/nmeth.4466 PubMed 29039415
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