CRISPR-StAR4BN (Lenti)
(Plasmid
#222692)
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Purpose(Empty Backbone) CRISPR-screening
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222692 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUW
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersNeomycin (select with G418) ; Blasticidin
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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MutationPGK without BsmBI cutsite
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CRISPR-StAR4BN (Lenti) was a gift from Ulrich Elling (Addgene plasmid # 222692 ; http://n2t.net/addgene:222692 ; RRID:Addgene_222692) -
For your References section:
CRISPR-StAR enables high-resolution genetic screening in complex in vivo models. Uijttewaal ECH, Lee J, Sell AC, Botay N, Vainorius G, Novatchkova M, Baar J, Yang J, Potzler T, van der Leij S, Lowden C, Sinner J, Elewaut A, Gavrilovic M, Obenauf A, Schramek D, Elling U. Nat Biotechnol. 2024 Dec 16. doi: 10.1038/s41587-024-02512-9. 10.1038/s41587-024-02512-9 PubMed 39681701