pLJM1-NRBP1-Cterm-N-FLAG
(Plasmid
#222678)
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Purposeoverexpressing C-terminus truncation of NRBP1 with N-terminal FLAG
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222678 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLJM1
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNRBP1
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SpeciesH. sapiens (human)
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MutationNRBP1 C-terminus truncation
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Entrez GeneNRBP1 (a.k.a. BCON3, MADM, MUDPNP, NRBP)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLJM1-NRBP1-Cterm-N-FLAG was a gift from Jason Moffat (Addgene plasmid # 222678 ; http://n2t.net/addgene:222678 ; RRID:Addgene_222678) -
For your References section:
The TSC22D, WNK, and NRBP gene families exhibit functional buffering and evolved with Metazoa for cell volume regulation. Xiao YX, Lee SY, Aguilera-Uribe M, Samson R, Au A, Khanna Y, Liu Z, Cheng R, Aulakh K, Wei J, Farias AG, Reilly T, Birkadze S, Habsid A, Brown KR, Chan K, Mero P, Huang JQ, Billmann M, Rahman M, Myers C, Andrews BJ, Youn JY, Yip CM, Rotin D, Derry WB, Forman-Kay JD, Moses AM, Pritisanac I, Gingras AC, Moffat J. Cell Rep. 2024 Jul 23;43(7):114417. doi: 10.1016/j.celrep.2024.114417. Epub 2024 Jul 8. 10.1016/j.celrep.2024.114417 PubMed 38980795