pQCXIB GFP (w301-1)
(Plasmid
#22266)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22266 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepQCXIB
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Backbone manufacturerEric Campeau
- Backbone size w/o insert (bp) 6869
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Vector typeMammalian Expression, Retroviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsTOP10F', 37oC
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEnhanced Green Fluorescent Protein
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Alt nameGFP
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Insert Size (bp)719
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site Eco RI (not destroyed)
- 5′ sequencing primer QCXIforw
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe GFP cDNA was derived from pQCXIP GFP (a gift from Dr. Peter Adams).
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQCXIB GFP (w301-1) was a gift from Eric Campeau (Addgene plasmid # 22266 ; http://n2t.net/addgene:22266 ; RRID:Addgene_22266)