TetR in pENTR1A (559-1)
(Plasmid #22265)

• Depositing Lab: Eric Campeau
• Publication: shMDC1 plasmids (unpublished)

Backbone

• Vector backbone: pENTR1A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2386
• Vector type: Mammalian Expression, Yeast Expression, Worm Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: TOP10F', 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tetracycline repressor
• Alt name: TetR
• Insert Size (bp): 1303
• Entrez Gene: tetR (a.k.a. SMR0223)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn I (not destroyed)
• 3′ cloning site: Eco RV (destroyed during cloning)
• 5′ sequencing primer: ENTRforw
• 3′ sequencing primer: ENTRrev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: TetR cDNA was derived from the pLenti 6/TR plasmid (Invitrogen).
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are several sequence discrepancies between Addgene's quality control sequence and the depositor's reference sequence. These changes do not affect plasmid function.