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pLV-miRFP680-NES-p2A-ezrin-mRuby3-IRES-Neo
(Plasmid #222636)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222636 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV-EF1a-IRES-Neo
  • Backbone size w/o insert (bp) 9211
  • Vector type
    Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miRFP680-NES-p2A-ezrin-mRuby3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3540
  • Entrez Gene
    EZR (a.k.a. CVIL, CVL, HEL-S-105, VIL2)
  • Promoter EF1a
  • Tag / Fusion Protein
    • mRuby3 (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-miRFP680-NES-p2A-ezrin-mRuby3-IRES-Neo was a gift from Arnold Hayer (Addgene plasmid # 222636 ; http://n2t.net/addgene:222636 ; RRID:Addgene_222636)

  • For your References section:

    Excitable Rho dynamics control cell shape and motility by sequentially activating ERM proteins and actomyosin contractility. Marshall-Burghardt S, Migueles-Ramirez RA, Lin Q, El Baba N, Saada R, Umar M, Mavalwala K, Hayer A. Sci Adv. 2024 Sep 6;10(36):eadn6858. doi: 10.1126/sciadv.adn6858. Epub 2024 Sep 6. 10.1126/sciadv.adn6858 PubMed 39241071
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