pLV-miRFP680-NES-p2A-ezrin-mRuby3-IRES-Neo
(Plasmid
#222636)
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PurposeEzrin activation reporter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 222636 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLV-EF1a-IRES-Neo
- Backbone size w/o insert (bp) 9211
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Vector typeLentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemiRFP680-NES-p2A-ezrin-mRuby3
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SpeciesH. sapiens (human)
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Insert Size (bp)3540
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Entrez GeneEZR (a.k.a. CVIL, CVL, HEL-S-105, VIL2)
- Promoter EF1a
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Tag
/ Fusion Protein
- mRuby3 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer EF-1a Forward
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-miRFP680-NES-p2A-ezrin-mRuby3-IRES-Neo was a gift from Arnold Hayer (Addgene plasmid # 222636 ; http://n2t.net/addgene:222636 ; RRID:Addgene_222636) -
For your References section:
Excitable Rho dynamics control cell shape and motility by sequentially activating ERM proteins and actomyosin contractility. Marshall-Burghardt S, Migueles-Ramirez RA, Lin Q, El Baba N, Saada R, Umar M, Mavalwala K, Hayer A. Sci Adv. 2024 Sep 6;10(36):eadn6858. doi: 10.1126/sciadv.adn6858. Epub 2024 Sep 6. 10.1126/sciadv.adn6858 PubMed 39241071
