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Addgene

pHIV-NAT-FLAG-CIP2A FL (sg2R)
(Plasmid #222624)

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Item Catalog # Description Quantity Price (USD)
Plasmid 222624 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pHIV-NAT-FLAG
  • Backbone manufacturer
    Ralph Scully
  • Backbone size w/o insert (bp) 7856
  • Total vector size (bp) 10574
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Nourseothricin (clonNAT)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CIP2A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2717
  • Entrez Gene
    CIP2A (a.k.a. KIAA1524, NOCIVA, p90)
  • Promoter EF-1-alpha promoter
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIV-NAT-FLAG-CIP2A FL (sg2R) was a gift from Daniel Durocher (Addgene plasmid # 222624 ; http://n2t.net/addgene:222624 ; RRID:Addgene_222624)

  • For your References section:

    The CIP2A-TOPBP1 axis safeguards chromosome stability and is a synthetic lethal target for BRCA-mutated cancer. Adam S, Rossi SE, Moatti N, De Marco Zompit M, Xue Y, Ng TF, Alvarez-Quilon A, Desjardins J, Bhaskaran V, Martino G, Setiaputra D, Noordermeer SM, Ohsumi TK, Hustedt N, Szilard RK, Chaudhary N, Munro M, Veloso A, Melo H, Yin SY, Papp R, Young JTF, Zinda M, Stucki M, Durocher D. Nat Cancer. 2021 Dec;2(12):1357-1371. doi: 10.1038/s43018-021-00266-w. Epub 2021 Nov 11. 10.1038/s43018-021-00266-w PubMed 35121901
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