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pHIV(T9)-DERA-sgresis
(Plasmid #222622)

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Item Catalog # Description Quantity Price (USD)
Plasmid 222622 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pHIV-EF1aT9
  • Backbone manufacturer
    Ralph Scully
  • Backbone size w/o insert (bp) 7808
  • Total vector size (bp) 8762
  • Modifications to backbone
    Quick change reaction allowed mutation of the TATAbox of the EF1a promoter. This mutation (tataa>CCCCC) is reported to decrease the transcription of the strong wild type promoter (DOI 10.1007/s11693-011-9089-0). Please note that because the NAT resistance gene is on an IRES with the GOI, NAT selection should be decreased to at least half the recommended dosage.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DERA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    984
  • Entrez Gene
    DERA (a.k.a. CGI-26, DEOC)
  • Promoter EF1a

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIV(T9)-DERA-sgresis was a gift from Daniel Durocher (Addgene plasmid # 222622 ; http://n2t.net/addgene:222622 ; RRID:Addgene_222622)

  • For your References section:

    Genome-scale mapping of DNA damage suppressors through phenotypic CRISPR-Cas9 screens. Zhao Y, Tabet D, Rubio Contreras D, Lao L, Kousholt AN, Weile J, Melo H, Hoeg L, Feng S, Cote AG, Lin ZY, Setiaputra D, Jonkers J, Gingras AC, Gomez Herreros F, Roth FP, Durocher D. Mol Cell. 2023 Aug 3;83(15):2792-2809.e9. doi: 10.1016/j.molcel.2023.06.025. Epub 2023 Jul 20. 10.1016/j.molcel.2023.06.025 PubMed 37478847
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