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pcDNA5-FRT/T0-Flag-BRIP1
(Plasmid #222619)

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Item Catalog # Description Quantity Price (USD)
Plasmid 222619 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pcDNA5 Flag-FRT-T0
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 5151
  • Total vector size (bp) 8896
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BRIP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3745
  • Entrez Gene
    BRIP1 (a.k.a. BACH1, FANCJ, OF)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-FRT/T0-Flag-BRIP1 was a gift from Daniel Durocher (Addgene plasmid # 222619 ; http://n2t.net/addgene:222619 ; RRID:Addgene_222619)

  • For your References section:

    Genome-scale mapping of DNA damage suppressors through phenotypic CRISPR-Cas9 screens. Zhao Y, Tabet D, Rubio Contreras D, Lao L, Kousholt AN, Weile J, Melo H, Hoeg L, Feng S, Cote AG, Lin ZY, Setiaputra D, Jonkers J, Gingras AC, Gomez Herreros F, Roth FP, Durocher D. Mol Cell. 2023 Aug 3;83(15):2792-2809.e9. doi: 10.1016/j.molcel.2023.06.025. Epub 2023 Jul 20. 10.1016/j.molcel.2023.06.025 PubMed 37478847
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