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pCAG_SRGAP2A_GAP(R527L_K566A)
(Plasmid #222617)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222617 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SRGAP2
  • Species
    H. sapiens (human)
  • Mutation
    Point mutations R527L and K566A (Rac1-specific GAP domain)
  • Entrez Gene
    SRGAP2 (a.k.a. ARHGAP34, FNBP2, SRGAP2A, SRGAP3)
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG_SRGAP2A_GAP(R527L_K566A) was a gift from Franck Polleux (Addgene plasmid # 222617 ; http://n2t.net/addgene:222617 ; RRID:Addgene_222617)

  • For your References section:

    SRGAP2 and Its Human-Specific Paralog Co-Regulate the Development of Excitatory and Inhibitory Synapses. Fossati M, Pizzarelli R, Schmidt ER, Kupferman JV, Stroebel D, Polleux F, Charrier C. Neuron. 2016 Jul 20;91(2):356-69. doi: 10.1016/j.neuron.2016.06.013. Epub 2016 Jun 30. 10.1016/j.neuron.2016.06.013 PubMed 27373832
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