pCAG_SRGAP2A_SH3(W765A)_GFP
(Plasmid
#222616)
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PurposeExpresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222616 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSRGAP2
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SpeciesH. sapiens (human)
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MutationPoint mutation W765A (SH3 domain)
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Entrez GeneSRGAP2 (a.k.a. ARHGAP34, FNBP2, SRGAP2A, SRGAP3)
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG_SRGAP2A_SH3(W765A)_GFP was a gift from Franck Polleux (Addgene plasmid # 222616 ; http://n2t.net/addgene:222616 ; RRID:Addgene_222616) -
For your References section:
SRGAP2 and Its Human-Specific Paralog Co-Regulate the Development of Excitatory and Inhibitory Synapses. Fossati M, Pizzarelli R, Schmidt ER, Kupferman JV, Stroebel D, Polleux F, Charrier C. Neuron. 2016 Jul 20;91(2):356-69. doi: 10.1016/j.neuron.2016.06.013. Epub 2016 Jun 30. 10.1016/j.neuron.2016.06.013 PubMed 27373832