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Addgene

pLenti X2 Blast/shp16 (w112-1)
(Plasmid #22261)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22261 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  • Backbone size w/o insert (bp) 7820
  • Vector type
    Lentiviral, RNAi
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3, 37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sh cyclin-dependent kinase inhibitor 2A
  • Alt name
    shp16
  • Insert Size (bp)
    61

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho I (destroyed during cloning)
  • 3′ cloning site Eco RI (not destroyed)
  • 5′ sequencing primer U6
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    shp16 was obtained from Dr. Christian Beausejour.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti X2 Blast/shp16 (w112-1) was a gift from Eric Campeau (Addgene plasmid # 22261 ; http://n2t.net/addgene:22261 ; RRID:Addgene_22261)
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