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Addgene

MAC3-C-GFP-NLS
(Plasmid #222604)

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Item Catalog # Description Quantity Price (USD)
Plasmid 222604 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    MAC3-tag-C (Addgene #185481)
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP and 5' nuclear localizaition sequence (NLS) (PPKTTRKVED)
  • Species
    Synthetic
  • Insert Size (bp)
    717
  • Promoter CMV
  • Tag / Fusion Protein
    • MAC3 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAC3-C-GFP-NLS was a gift from Markku Varjosalo (Addgene plasmid # 222604 ; http://n2t.net/addgene:222604 ; RRID:Addgene_222604)

  • For your References section:

    Analyzing Protein Interactions by MAC-Tag Approaches. Liu X, Salokas K, Keskitalo S, Martinez-Botia P, Varjosalo M. Methods Mol Biol. 2023;2690:281-297. doi: 10.1007/978-1-0716-3327-4_24. 10.1007/978-1-0716-3327-4_24 PubMed 37450155
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