pLenti CMV p16 Neo (w111-1)
(Plasmid #22260)

• Depositing Lab: Eric Campeau
• Publication: shMDC1 plasmids (unpublished)

Backbone

• Vector backbone: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
• Backbone size w/o insert (bp): 8221
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3, 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cyclin-dependent kinase inhibitor 2A
• Alt name: p16
• Species: H. sapiens (human)
• Insert Size (bp): 447
• Entrez Gene: CDKN2A (a.k.a. ARF, CAI2, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal I (not destroyed)
• 3′ cloning site: Xho I (not destroyed)
• 5′ sequencing primer: CMVforw
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: p16 cDNA obtained from Dr. Christian Beausejour.
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry